Catherine Tate, who has herself starred in Doctor Who in the past, will perform a skit as her famous schoolgirl character Lauren, from The Catherine Tate Show.
The video will also include contributions from past Doctors including Tom Baker, Sylvester McCoy, Peter Davison and Paul McGann.
Doctor Who stars Jodie Whittaker, Matt Smith, David Tennant and Peter Capaldi have teamed up to send a message of appreciation for the work of the NHS.
The Big Night In airs on BBC One at 7pm on Thursday, 23 April 2020.