The song playing is, of course, Survivor’s monster hit “Eye of the Tiger,” which gained international fame as the theme from Sylvester Stallone’s Rocky III.
The Season 4 episode “Yellow Fever” featured a scene in which Jared Padalecki’s Sam found Ackles’ Dean listening to the track in the show’s signature Impala.
And while it’s countless times across the pop culture-scape, the song definitely holds a special place in the ears and hearts of Supernatural fans.
More famously, however, the episode ends on Ackles’ bonkers outtake in which he lip syncs the shit out of “Eye of the Tiger” while also showing off his air-drumming and car-climbing skills.