Season 1 was something of a prequel to the comics, taking place entirely in Annville, the small Texas town where Jesse is a Preacher.
The trailer is still very much a tease, lasting just 30 seconds, but includes several entirely new scenes.
Preacher Season 2 premieres on Sunday, June 25 before moving to its regular time on Mondays for the rest of the season.
Fans of the books know that the Cowboy is, in fact, the Saint of Killers, an immortal, supernatural killing machine who has been sent after Jesse (and Genesis within him).