She has feelings and independence now, and how many of us wouldn’t at least want to lash out if somebody tried to knock us out and then start talking about ways to improve us?
A.I.D.A. has evidently had enough of hiding her awareness from the humans around her, and their intention to deactivate her pushed her to attack.
Radcliffe’s move to put her on pause to chat with Fitz about ways to upgrade her software probably didn’t make her feel any better about them.
Sure, we might not physically attack and initiate a plot to overthrow any establishments, but it’s not difficult to see why A.I.D.A. was sick of faking her obedience and is ready to go rogue.